Imine reductase (IRED)
Kufotokozera Kwachidule:
About Ine reductase
ES-IRED: Gulu la ma enzyme omwe amalimbikitsa kuchepetsa kwa C=N bond ku CN bond. Ndi la redox enzyme, yokhala ndi coenzyme NADPH yoyendetsera hydrogen. Malinga ndi zomwe chiral amakonda, amatha kugawidwa m'magulu a R-IRED ndi S-IRED. Syncozymes adapanga ma imine reductase 15 (numbIRED ES-IRED-101-ES-IRED-115), omwe angagwiritsidwe ntchito kwambiri pochepetsa regioselective ndi stereoselective ya imines kuti akonze ma chiral amines.
Mtundu wa catalytic reaction:
Foni yam'manja/Wechat/WhatsApp: +86-13681683526
Imelo:lchen@syncozymes.com
| Ma enzyme | Khodi ya Zamalonda | Kufotokozera |
| Ufa wa Enzyme | ES-IRED-101~ ES-IRED-114 | seti ya 14 Imine Reductase, 50 mg iliyonse |
| Kiti Yowunikira Enzyme ya Zitsime 96 | ES-IRED-1400 | seti ya 14 Imine Reductase, 1 mg iliyonse |
★ Kuchuluka kwa substrate.
★ Kusankha bwino kwa chiral.
★ Kusintha kwakukulu.
★ Zogulitsa zochepa.
★ Zinthu zofatsa zomwe zimachitika chifukwa cha kukwiya.
★ Yosamalira chilengedwe.
➢ Kawirikawiri, dongosolo la reaction liyenera kukhala ndi substrate, buffer solution, enzyme, coenzyme, ndi coenzyme regeneration system.
➢ Mitundu yonse ya ma ES-IRED omwe ali ndi machitidwe osiyanasiyana abwino kwambiri ayenera kuphunziridwa payekhapayekha.
➢ Kuchuluka kwa zinthu zomwe zili ndi substrate kapena mankhwala ena kungalepheretse ntchito ya ES-IRED. Komabe, kuletsa kumeneku kungachepetsedwe powonjezera substrate.
Chitsanzo 1 (Kupanga kwa chiral 2-methyl pyrrolidine)(1):
Chitsanzo 2 (Kupanganso kwa amine yachiwiri)(2):
Chitsanzo 3 (Kuchepetsa Migodi Yozungulira)(3):
Sungani kwa zaka ziwiri pansi pa -20℃.
Musagwirizane ndi zinthu zoopsa monga: kutentha kwambiri, pH yapamwamba/yotsika komanso organic solvent yochuluka.
1. Scheller PN, Fademrecht S, Hofelzer S, et al. ChemBioChem, 2014, 15, 2201-2204.
2. Wetzl D, Gand M, Ross A, ndi ena. ChemCatChem, 2016, 8, 2023-2026.
3. Li H, Luan ZJ, Zheng GW, et al. Adv. Synth. Catal. 2015, 357, 1692-1696.
