Lactate Dehydrogenase (LDH)
Kufotokozera Kwachidule:
Zokhudza Lactate dehydrogenase
Pali mtundu umodzi wa LDH enzyme product (Nambala monga ES-LDH) yopangidwa ndi SyncoZymes. LDH imatha kuyambitsa kuchepa kwa pyruvate kukhala L-lactic acid, kapena kuchepetsa ma α-keto acid ena ofanana. Mu catalysis, NADH ndi yofunika ngati chonyamulira hydrogen.
Lactate dehydrogenase mtundu wa catalytic reaction
Foni yam'manja/Wechat/WhatsApp: +86-13681683526
Imelo:lchen@syncozymes.com
| Ma enzyme | Khodi ya Zamalonda | Kufotokozera |
| Ufa wa Enzyme | ES-LDH-101 | seti ya 1 Monoamine Oxidase, 50 mg iliyonse 1 chinthu * 50mg / chinthu, kapena kuchuluka kwina |
Njira yotsatirayi yochitira zinthu ingagwiritsidwe ntchito pofotokoza:
1-10 mg/ml: Gawo lapansi
10 mg/ml: Enzyme
10% (v/v): Chosungunuka chachilengedwe (1)
100 mM: Phosphate buffer (pH7.0)
0.2 mg/ml: NAD+
100 mM: Ammonium formate
10 mg/ml: Formate dehydrogenase (FDH)
Ikani zinthuzo mu chivundikiro cha kutentha chokhazikika (monga 30oC, 150rpm) kwa maola 24 mpaka 48. Ikani mu centrifuge chisakanizo chilichonse kuti chikhale ndi mapuloteni okwanira, ndipo supernatant ingagwiritsidwe ntchito pa TLC, HPLC kapena GC analysis.
Chidziwitso (1): Zosungunulira zachilengedwe (monga DMSO, methanol, acetonitrile, IPA, ndi zina zotero) ndizosankha, zomwe zimagwiritsidwa ntchito pazinthu zosasungunuka.
