Esterase&Lipase (PLE&CALB)
Pali mitundu 26 ya ma enzyme a PLE (Nambala monga ES-PLE-101～ES-PLE-126) yopangidwa ndi SyncoZymes.ES-PLE ikhoza kugwiritsidwa ntchito popanga hydrolysis ya aliphatic ndi ester compounds, kapena regioselective ndi stereoelective resolution kuti apange chiral acid ndi zotumphukira zake.
Mtundu wa Catalytic reaction:
★ Kukhazikika kwa gawo lapansi.
★ Kusankha kwamphamvu kwachiral.
★ Kutembenuka kwakukulu.
★ Zogulitsa zochepa.
★ Mkhalidwe wofatsa.
★ Okonda zachilengedwe.
➢ Nthawi zambiri, kachitidwe kachitidwe kamayenera kukhala ndi gawo lapansi, yankho la buffer ndi ES-PLE.Kukhazikika kwa ma ES-PLE ena kumachitika mu gawo lachilengedwe.
➢ Mitundu yonse ya ma ES-PLE ogwirizana ndi momwe zinthu ziliri bwino ziyenera kuphunziridwa payekhapayekha.
Chitsanzo 1 (Biosynthesis of Pregabalin Intermediate)(1):
Chitsanzo 2(2):
Chitsanzo 3(3):
Chitsanzo 4(4):
Sungani zaka 2 pansi -20 ℃.
Osakumana ndi zovuta kwambiri monga: kutentha kwambiri, kutsika / kutsika pH.
1. Xu FX, Chen SY, Xu G, ndi tal.Appl.Biotechnol Bioproc E, 1988, 54(4): 1030.
2. Huang, FC, Lee, LF, Mittal, RSD e tal.J. Am.Chem.Soc, 1975, 97, 4144.
3. Kielbasinski, P., Goralczyk, P., Mikolajczyk, M., e tal.Synlett, 1994, 127.
4. Gais, HJ, Griebel, C., Buschmann, H. Tetrahedron:Asymmetry 2000, 11, 917
