Hydroaminase (HAM)
Kufotokozera Kwachidule:
Zambiri za Hydroaminase
Pali mitundu iwiri ya enzyme ya HAM (Nambala monga ES-HAM-101~ ES-HAM-102) yopangidwa ndi SyncoZymes.HAM imatha kuyambitsa ammoniation ya enoic acid kapena zotumphukira zake kuti apange chiral amino acid.HAM ingagwiritsidwe ntchito kupanga ma amino acid (kapena chiral amines) kuchokera ku enoic acid (kapena alkenes).Wopereka ammonia, monga ammonia ammonia kapena ammonium mchere, ndizofunikira.
Hydroaminase catalytic reaction mtundu
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Imelo:lchen@syncozymes.com
| Ma enzyme | Kodi katundu | Kufotokozera |
| Enzyme Powder | ES-HAM-101~ ES-HAM-102 | seti ya 2 Nuclosidephosphoryalse, 50 mg chilichonse 2 zinthu * 50mg / chinthu, kapena kuchuluka kwina |
Njira zotsatirazi zitha kugwiritsidwa ntchito pofotokoza:
1-10 mg/ml: gawo lapansi
10 mg/ml: Enzyme
10% (v/v): organic cosolvent (1)
10% (v/v): Ammonia
Sinthani pH kukhala 9.0 musanawonjezere enzyme, ndipo enzyme iyenera kuwonjezeredwa pomaliza.Ikani zomwe zikuchitika pa kutentha kosasinthasintha (mwachitsanzo, 30oC, 150rpm) kwa maola 24-48.Centrifuge aliyense osakaniza kuti precipitate mapuloteni, iwo supernatant angagwiritsidwe ntchito TLC, HPLC kapena GC kusanthula.
Zindikirani (1): Zosungunulira za organic (monga DMSO, methanol, acetonitrile, IPA, etc.) ndizosankha, zomwe zimagwiritsidwa ntchito popanga magawo osasungunuka.
