Leucine Dehydrogenase (LeuDH)
Kufotokozera Kwachidule:
Zokhudza Leucine dehydrogenase
Pali mtundu umodzi wa mankhwala a enzyme a LeuDH (Nambala monga ES-LeuDH-101) omwe adapangidwa ndi SyncoZymes. LeuDH imatha kuyambitsa ammoniation yochepetsa mphamvu ya 4-methylbutanoic acid (mchere) kupita ku L-leucine kapena okosijeni ya leucine kupita ku 4-methylbutanoic acid. ES-LeuDH imathanso kuyambitsa mphamvu yochepetsa mphamvu ya α-keto acids yokhala ndi kapangidwe kofanana kuti ikonzekere zotumphukira za amino acid zofanana. Mu njira ya catalysis, NADH ndiyofunikira ngati chonyamulira hydrogen.
Leucine dehydrogenase mtundu wa catalytic reaction
Foni yam'manja/Wechat/WhatsApp: +86-13681683526
Imelo:lchen@syncozymes.com
| Ma enzyme | Khodi ya Zamalonda | Kufotokozera |
| Ufa wa Enzyme | ES-LeuDH-101 | seti ya 1 Monoamine Oxidase, 50 mg iliyonse 1 chinthu * 50mg / chinthu, kapena kuchuluka kwina |
Njira yotsatirayi yochitira zinthu ingagwiritsidwe ntchito pofotokoza:
1-10 mg/ml: Gawo lapansi
10 mg/ml: Enzyme
10% (v/v): Chosungunuka chachilengedwe (1)
100 mM: Phosphate buffer (pH7.0)
0.2 mg/ml: NAD+
100 mM: Ammonium formate
10 mg/ml: Formate dehydrogenase (FDH)
Ikani zinthuzo mu chivundikiro cha kutentha chokhazikika (monga 30oC, 150rpm) kwa maola 24 mpaka 48. Ikani mu centrifuge chisakanizo chilichonse kuti chikhale ndi mapuloteni okwanira, ndipo supernatant ingagwiritsidwe ntchito pa TLC, HPLC kapena GC analysis.
Chidziwitso (1): Zosungunulira zachilengedwe (monga DMSO, methanol, acetonitrile, IPA, ndi zina zotero) ndizosankha, zomwe zimagwiritsidwa ntchito pazinthu zosasungunuka.
