Leucine Dehydrogenase (LeuDH)
Kufotokozera Kwachidule:
Za Leucine dehydrogenase
Pali mtundu umodzi wa enzyme ya LeuDH (Nambala ngati ES-LeuDH-101) yopangidwa ndi SyncoZymes.LeuDH ikhoza kuyambitsa reductive ammoniation ya 4-methylbutanoic acid (mchere) ku L-leucine kapena oxidation ya leucine ku 4-methylbutanoic acid.ES-LeuDH imathanso kuthandizira kuchepetsa ma amino acid a α-keto okhala ndi mawonekedwe ofanana kuti akonzekere zotengera za amino acid.Panthawi ya catalysis, NADH ndiyofunikira ngati hydrogen transporter.
Leucine dehydrogenasecatalytic reaction type
Mobile/Wechat/WhatsApp: +86-13681683526
Imelo:lchen@syncozymes.com
| Ma enzyme | Kodi katundu | Kufotokozera |
| Enzyme Powder | ES-LeuDH-101 | seti ya 1 Monoamine Oxidase, 50 mg chilichonse 1 zinthu * 50mg / chinthu, kapena kuchuluka kwina |
Njira zotsatirazi zitha kugwiritsidwa ntchito pofotokoza:
1-10 mg/ml: gawo lapansi
10 mg/ml: Enzyme
10% (v/v): organic cosolvent (1)
100 mM: Phosphate buffer (pH7.0)
0.2 mg/ml: NAD+
100 mM: Ammonium formate
10 mg/ml: Pangani dehydrogenase (FDH)
Ikani zomwe zikuchitika pa kutentha kosasinthasintha (mwachitsanzo, 30oC, 150rpm) kwa maola 24-48.Centrifuge chosakaniza chilichonse kuti chiwongolere mapuloteni, iwo omwe ali apamwamba amatha kugwiritsidwa ntchito pakuwunika kwa TLC, HPLC kapena GC.
Zindikirani (1): Zosungunulira za organic (monga DMSO, methanol, acetonitrile, IPA, etc.) ndizosankha, zomwe zimagwiritsidwa ntchito popanga magawo osasungunuka.
