CALB yosasunthika

Kufotokozera Kwachidule:

Chithunzi cha CALB

Recombinant Lipase B yochokera ku Candida antarctica (CALB) imapangidwa ndi kuwira pansi pamadzi ndi Pichia pastoris yosinthidwa chibadwa.

CALB angagwiritsidwe ntchito mu gawo madzi kapena organic gawo chothandizira esterification, esterolysis, transesterification, mphete kutsegula poliyesitala kaphatikizidwe, aminolysis, hydrolysis wa amides, acylation wa amines ndi zina anachita.

CALB ili ndi high chiral selectivity ndi kusankha udindo, kotero ingagwiritsidwe ntchito kwambiri pokonza mafuta, chakudya, mankhwala, zodzoladzola ndi mafakitale ena a mankhwala.

Mobile/Wechat/WhatsApp: +86-13681683526

Imelo:lchen@syncozymes.com

Titumizireni imelo

Tsatanetsatane wa Zamalonda

Zogulitsa Tags

CALB Yosayenda:

CALB imakhala yosasunthika ndi kutengeka kwa thupi pa utomoni wapamwamba kwambiri wa hydrophobic womwe ndi macroporous, styrene / methacrylate polima.Immobilized CALB ndiyoyenera kugwiritsidwa ntchito mu zosungunulira za organic ndi makina opanda zosungunulira, ndipo imatha kubwezeredwa ndi kugwiritsidwanso ntchito kwanthawi zambiri pamikhalidwe yoyenera.
Khodi ya malonda: SZ-CALB- IMMO100A, SZ-CALB- IMMO100B.

Ubwino wa SZ-CALB-IMMO100:

★Zochita zapamwamba, kusankha kwapamwamba kwambiri komanso kukhazikika kwapamwamba.
★Kuchita bwino m'magawo opanda madzi.
★Chotsani mosavuta pamachitidwe, thetsani zomwe zikuchitika, ndipo pewani zotsalira za mapuloteni muzinthu.
★Itha kusinthidwanso ndi kugwiritsidwanso ntchito kuti muchepetse mtengo.

Zogulitsa za SZ-CALB-IMMO100:

Zochita	≥10000PLU/g
Mtundu wa pH kuti muyankhe	5-9
Kutentha kwa zosiyanasiyana	10-60 ℃
Maonekedwe	CALB-IMMO100-A: Yellow yellow to bulauni wolimba CALB-IMMO100-B: Choyera mpaka bulauni cholimba
Tinthu kukula	300-500μm
Kutaya pakuyanika pa 105 ℃	0.5% -3.0%
Resin kwa immobilization	Macroporous, styrene/methacrylate polima
Zosungunulira zochita	Madzi, organic zosungunulira, etc., kapena popanda zosungunulira.Pazochita zina zosungunulira organic, 3% madzi akhoza kuwonjezeredwa kuti apititse patsogolo zotsatira zake
Tinthu kukula	CALB-IMMO100-A: 200-800 μm CALB-IMMO100-B: 400-1200 μm

Tanthauzo la unit: 1 unit ikufanana ndi kaphatikizidwe ka 1μmol pamphindi propyl laurate kuchokera ku asidi lauric ndi 1-propanol pa 60 ℃.Zomwe zili pamwambazi za CALB-IMMP100-A ndi CALB-IMMO100-B zimagwirizana ndi zonyamulira zopanda mphamvu zokhala ndi tinthu tating'ono tosiyanasiyana.

Malangizo ndi Chitetezo:

1. Mtundu wa reactor
The immobilized enzyme imagwira ntchito ku ketulo batch reactor ndi bedi lokhazikika loyenda mosalekeza.Tiyenera kuzindikira kuti kupewa kuphwanya chifukwa cha mphamvu yakunja panthawi yodyetsa kapena kudzaza.
2. Kuchita pH, kutentha ndi zosungunulira
Enzyme yosasunthika iyenera kuwonjezeredwa komaliza, zinthu zina zitawonjezeredwa ndikusungunuka, ndikusintha pH.
Ngati kumwa kwa gawo lapansi kapena kupanga zinthu kungayambitse kusintha kwa pH panthawi yakuchita, chotchinga chokwanira chiyenera kuwonjezeredwa pamachitidwe, kapena pH iyenera kuyang'aniridwa ndikusinthidwa panthawi yomwe akuchita.
Mkati mwa kulekerera kutentha kwa CALB (pansi pa 60 ℃), kutembenuka kumawonjezeka ndi kuwonjezeka kwa kutentha.Pogwiritsira ntchito, kutentha kwazomwe ziyenera kusankhidwa molingana ndi kukhazikika kwa gawo lapansi kapena mankhwala.
Nthawi zambiri, ester hydrolysis reaction ndi oyenera mu amadzimadzi gawo dongosolo, pamene ester kaphatikizidwe anachita ndi oyenera dongosolo organic gawo.Zosungunulira zachilengedwe zimatha kukhala ethanol, tetrahydrofuran, n-hexane, n-heptane ndi toluene, kapena zosungunulira zosakaniza zoyenera.Pazochita zina zosungunulira organic, 3% madzi akhoza kuwonjezeredwa kuti apititse patsogolo zotsatira zake.
3. Kugwiritsanso ntchito ndi moyo wantchito wa CALB
Pansi pamachitidwe oyenera, CALB imatha kubwezeretsedwanso ndikugwiritsidwanso ntchito, ndipo nthawi zogwiritsira ntchito zimasiyana ndi mapulojekiti osiyanasiyana.
Ngati CALB yopezedwayo sigwiritsidwanso ntchito mosalekeza ndipo ikufunika kusungidwa ikachira, iyenera kutsukidwa ndikuumitsidwa ndikusindikizidwa pa 2-8 ℃.
Pambuyo pakugwiritsanso ntchito kangapo, ngati magwiridwe antchito achepetsedwa pang'ono, CALB ikhoza kuwonjezeredwa moyenera ndikupitilizabe kugwiritsa ntchito.Ngati momwe zimagwirira ntchito zimachepetsedwa kwambiri, ziyenera kusinthidwa.

Zitsanzo za Ntchito:

Chitsanzo 1(Aminolysis)⁽¹⁾:

Chitsanzo 2(Aminolysis)⁽²⁾:

Chitsanzo 3 (Kutsegula mphete ya polyester synthesis)⁽³⁾:

Chitsanzo 4(Transesterification, regioselective of hydroxyl group)⁽⁴⁾:

Chitsanzo 5(Transesterification, Kinetic kuthetsa mowa wa racemic)⁽⁵⁾:

Chitsanzo 6 (Esterification, kinetic resolution ya carboxylic acid)⁽⁶⁾:

Chitsanzo 7 (Esterolysis, Kinetic resolution)⁽⁷⁾:

Chitsanzo 8(Hydrolysis of amides)⁽⁸⁾:

Chitsanzo 9(Acylation of Amines)⁽⁹⁾:

Chitsanzo 10(Aza-Michael addition reaction)⁽¹⁰⁾:

Zolozera:

1. Chen S, Liu F, Zhang K, ndi tal.Tetrahedron Lett, 2016, 57: 5312-5314.
2. Olah M, Boros Z, anszky GH, e tal.Tetrahedron, 2016, 72: 7249-7255.
3. Nakaoki1 T, Mei Y, Miller LM, e tal.Ind. Biotechnol, 2005, 1(2):126-134.
4. Pawar SV, Yadav G DJ Ind. Eng.Chem, 2015, 31: 335-342.
5. Kamble MP, Shinde SD, Yadav G DJ Mol.Catal.B: Enzym, 2016, 132: 61-66.
6. Shinde SD, Yadav G D. Process Biochem, 2015, 50: 230-236.
7. Souza TC, Fonseca TS, Costa JA, ndi tal.J. Mol.Catal.B: Enzym, 2016, 130: 58-69.
8. Gavil'an AT, Castillo E, Lopez-Mungu'AJ Mol.Catal.B: Enzym, 2006, 41: 136-140.
9. Joubioux FL, Henda YB, Bridiau N, ndi tal.J. Mol.Catal.B: Enzym, 2013, 85-86: 193-199.
10. Dhake KP, Tambade PJ, Singhal RS, e tal.Tetrahedron Lett, 2010, 51: 4455-4458.