D-Amino acid dehydrogenase (D-AADH)
Kufotokozera Kwachidule:
Zokhudza D-Amino acid dehydrogenase
ES-D-AADH (D-Amino acid dehydrogenase): enzyme yomwe imatha kuchepetsa 2-keto acid kukhala D-amino acid yofanana. Zomwe zimachitika zimafuna NADP(H) ndi inorganic ammonia donor (monga ammonia kapena ammonium ion). Pali mitundu 17 ya D-amino acid dehydrogenases (Nambala monga ES-DAADH-101~ ES-DAADH-117) yopangidwa ndi SyncoZymes.
Mtundu wa catalytic reaction:
Foni yam'manja/Wechat/WhatsApp: +86-13681683526
Imelo:lchen@syncozymes.com
| Ma enzyme | Khodi ya Zamalonda | Kufotokozera |
| Ufa wa Enzyme | ES-D-AADH-101~ ES-D-AADH-117 | seti ya 17 D-amino Acid Dehydrogenases, 50 mg iliyonse 17 zinthu * 50mg / chinthu, kapena kuchuluka kwina |
| Zida Zowunikira (SynKit) | ES-D-AADH-1700 | seti ya 17 D-amino Acid Dehydrogenases, 1 mg iliyonse Zinthu 17 * 1mg / chinthu |
★ Kuchuluka kwa substrate.
★ Kusankha bwino kwa chiral.
★ Kusintha kwakukulu.
★ Zogulitsa zochepa.
★ Zinthu zofatsa zomwe zimachitika chifukwa cha kukwiya.
★ Yosamalira chilengedwe.
➢ Kawirikawiri, njira yochitira zinthu iyenera kukhala ndi gawo lapansi, yankho la buffer, enzyme, coenzyme ndi coenzyme regeneration system (monga shuga ndi glucose dehydrogenase).
➢ D-AADH iyenera kuwonjezeredwa komaliza mu dongosolo la reaction, pH ndi kutentha zitasinthidwa kuti zigwirizane ndi momwe reaction imakhalira.
Chitsanzo 1 (Kuchepetsa α Ketoacids kukhala D-amino acids yofanana)(1):
Sungani kwa zaka ziwiri pansi pa -20℃.
Musagwirizane ndi zinthu zoopsa monga: kutentha kwambiri, pH yapamwamba/yotsika komanso organic solvent yochuluka.
1. Akita H, Hayashi J, Sakuraba H, ndi ena. Frontiers in Microbiology, 2018, 9: 1760.
