Cytochrome P450 monooxygenase (CYP)

Kufotokozera Kwachidule:

Zokhudza CYP

ES-CYPs: Gulu la ma enzyme a redox omwe ali ndi mercaptan-heme ngati malo ogwirira ntchito poyambitsa kusamutsa mpweya. Ndi ma terminal oxygenases ndipo amafunika heme kuti ayambitse mpweya.

Cytochrome P450 monooxygenase imayambitsa okosijeni, epoxidation ndi hydroxylation ya alkyl, hydroxylation ndi okosijeni ya ammonia, okosijeni ya sulfure, dealkylation (oxygen, sulfure, ammonia), okosijeni dehydrogenation, deamination ndi dehalogenation.
Pali mitundu 8 ya zinthu za cytochrome P450 monooxygenase (Nambala monga ES-CYP-101～ES-CYP-108) zopangidwa ndi SyncoZymes.

Foni yam'manja/Wechat/WhatsApp: +86-13681683526

Imelo:lchen@syncozymes.com

Tumizani imelo kwa ife

Tsatanetsatane wa Zamalonda

Ma tag a Zamalonda

Zokhudza CYP:

Mtundu wa catalytic reaction:

Zambiri Zamalonda:

Ma enzyme	Zida Zowunikira (SynKit)	Kufotokozera
Ufa wa Enzyme	ES-CYP-101~ ES-CYP-108	seti ya ma Cytochrome P450 Monooxygenases 8, 50 mg iliyonse zinthu 8 * 50mg / chinthu, kapena kuchuluka kwina
Zida Zowunikira (SynKit)	ES-CYP-800	seti ya ma Cytochrome P450 Monooxygenases 8, 1mg iliyonse Zinthu 8 * 1mg / chinthu

Ubwino:

★ Mtundu wa substrate wokulirapo.
★ Kusintha kwakukulu.
★ Zogulitsa zochepa.
★ Zinthu zofatsa zomwe zimachitika chifukwa cha kukwiya.
★ Yosamalira chilengedwe.

Malangizo ogwiritsira ntchito:

➢ Kawirikawiri, dongosolo la reaction liyenera kukhala ndi substrate, buffer solution (The optimum reaction pH of enzyme), coenzyme (NAD(H) kapena NADP(H)), coenzyme regeneration system (monga glucose ndi glucose dehydrogenase) ndi ES-CYP. coenzyme ndi coenzyme regeneration system zitha kusinthidwa ndi hydrogen peroxide mu gawo la reaction system.
➢ Mitundu yonse ya ma ES-CYPs omwe amagwirizana ndi machitidwe osiyanasiyana abwino a reaction ayenera kuphunziridwa payekhapayekha.
➢ Kuchuluka kwa substrate kapena mankhwala omwe ali ndi substrate kungalepheretse ntchito ya ES-CYP. Komabe, kuletsako kungachepetsedwe powonjezera substrate.

Zitsanzo za Kugwiritsa Ntchito:

Chitsanzo 1⁽¹⁾:

Chitsanzo 2⁽²⁾:

Chitsanzo 3⁽³⁾:

Chitsanzo 4⁽⁴⁾:

Malo Osungira:

Sungani kwa zaka ziwiri pansi pa -20℃.

Chenjezo:

Musagwirizane ndi zinthu zoopsa monga: kutentha kwambiri, pH yapamwamba/yotsika komanso organic solvent yochuluka.

Maumboni:

1. Zaretzki J, Matlock M, ndi Swamidass S JJ Chem. Inf. Model, 2013, 53, 3373–3383.
2. Gannett P M., Kabulski J, Perez F A., ndi tal. J. Am. Chemu. Soc., 2006, 128 (26), 8374-8375.
3. Cryle M J., Matovic N J., ndi De Voss J J. Org. Lett., 2003, 5 (18), 3341–3344.
4. Kawauchi, H., Sasaki, J., Adachi, T., e tal. Biochim. Biophys. Acta, 1994, 1219, 179.
5. Yasutake, Y., Fujii, Y.; Cheon, WK ndi tal. Chithunzi cha Acta Crystallog. 2009, 65, 372.