Cytochrome P450 monooxygenase (CYP)

Kufotokozera Kwachidule:

Za CYP

ES-CYPs: Kalasi ya ma enzymes a redox okhala ndi mercaptan-heme ngati malo ogwirira ntchito kuti athandizire kutumiza mpweya.Ndiwo ma oxygenases omaliza ndipo amafunikira heme kuti ayambitse mpweya.

Cytochrome P450 monooxygenase imathandizira makutidwe ndi okosijeni, epoxidation ndi hydroxylation ya alkili, hydroxylation ndi oxidation ya ammonia, okosijeni wa sulfure, dealkylation (oxygen, sulfure, ammonia), oxidative dehydrogenation, deamination ndi dehalogenation.
Pali mitundu 8 ya zinthu za cytochrome P450 monooxygenase (Nambala ngati ES-CYP-101～ES-CYP-108) zopangidwa ndi SyncoZymes.

Mobile/Wechat/WhatsApp: +86-13681683526

Imelo:lchen@syncozymes.com

Titumizireni imelo

Tsatanetsatane wa Zamalonda

Zogulitsa Tags

Za CYP:

Mtundu wa Catalytic reaction:

Zambiri Zamalonda:

Ma enzyme	Screening Kit (SynKit)	Kufotokozera
Enzyme Powder	ES-CYP-101~ ES-CYP-108	seti ya 8 Cytochrome P450 Monooxygenases, 50 mg chilichonse 8 zinthu * 50mg / chinthu, kapena kuchuluka kwina
Screening Kit (SynKit)	ES-CYP-800	seti ya 8 Cytochrome P450 Monooxygenases, 1mg chilichonse 8 zinthu * 1mg / chinthu

Ubwino:

★ Kutalikirana kwa gawo lapansi.
★ Kutembenuka kwakukulu.
★ Zogulitsa zochepa.
★ Mkhalidwe wofatsa.
★ Okonda zachilengedwe.

Malangizo ogwiritsira ntchito:

➢ Nthawi zambiri, kachitidwe kachitidwe kamayenera kukhala ndi gawo lapansi, yankho la buffer (The optimum reaction pH of enzyme), coenzyme (NAD(H) kapena NADP(H)), coenzyme regeneration system (mwachitsanzo shuga ndi glucose dehydrogenase) ndi ES-CYP.coenzyme ndi coenzyme regeneration system zitha kusinthidwa ndi hydrogen peroxide mu dongosolo lakuchita pang'ono.
➢ Mitundu yonse ya ma ES-CYPs ofanana ndi momwe zinthu ziliri bwino ziyenera kuphunziridwa payekhapayekha.
➢ Highconcentration Substrate kapena mankhwala omwe ali ndi zinthu akhoza kulepheretsa ES-CYP ntchito.Komabe, zoletsazo zitha kumasulidwa ndikuwonjezera batch ya gawo lapansi.

Zitsanzo za Ntchito:

Chitsanzo 1⁽¹⁾:

Chitsanzo 2⁽²⁾:

Chitsanzo 3⁽³⁾:

Chitsanzo 4⁽⁴⁾:

Posungira:

Sungani zaka 2 pansi -20 ℃.

Chenjerani:

Osakhudzana ndi zinthu zowopsa monga: kutentha kwambiri, kutsika / kutsika pH komanso zosungunulira za organic.

Zolozera:

1. Zaretzki J, Matlock M, ndi Swamidass S JJ Chem.Inf.Chitsanzo, 2013, 53, 3373-3383.
2. Gannett P M., Kabulski J, Perez F A., ndi tal.J. Am.Chemu.Soc., 2006, 128 (26), 8374-8375.
3. Cryle M J., Matovic N J., ndi De Voss J J. Org.Lett., 2003, 5 (18), 3341-3344.
4. Kawauchi, H., Sasaki, J., Adachi, T., e tal.Biochim.Biophys.Acta, 1994, 1219, 179.
5. Yasutake, Y., Fujii, Y.;Cheon, WK ndi tal.Chithunzi cha Acta Crystallog.2009, 65, 372.