Mowa wochuluka (AOX)
Kufotokozera Kwachidule:
Zokhudza mowa oxidase
ES-AOXs: Ma enzyme amenewa amatha kuyambitsa okosijeni wa mafuta ochulukirapo kapena aryl-alcohols kuti apange aldehydes. Amagwiritsa ntchito okosijeni wa mamolekyu koma safuna cofactor yowonjezerapo, ndi hydrogen peroxide yopangidwa.
Pali mitundu 6 ya zinthu zopangidwa ndi mowa (Nambala monga ES-AOX-101~ES-AOX-106) zomwe zapangidwa ndi SyncoZymes. ES-AOX101 ndi ES-AOX102 amakonda zinthu zopangidwa ndi mowa wa aliphatic, ES-AOX103~ ES-AOX105 amakonda zinthu zopangidwa ndi mowa wa aromatic, ndipo ES-AOX106 ndi cholesterol oxidase. SZ-AOX ndi chida chothandiza chothandizira kusungunuka kwa mafuta a alcohols kapena aryl-alcohols kuti apange aldehydes.
Mtundu wa catalytic reaction:
Foni yam'manja/Wechat/WhatsApp: +86-13681683526
Imelo:lchen@syncozymes.com
| Ma enzyme | Khodi ya Zamalonda | Kufotokozera |
| Ufa wa Enzyme | ES-AOX-101~ ES-AOX-105 | seti ya ma oxidases 5 a mowa, 50 mg iliyonse Zinthu 5 * 50mg / chinthu, kapena kuchuluka kwina |
| Zida Zowunikira (SynKit) | ES-AOX-500 | seti ya ma oxidases 5 a mowa, 50 mg iliyonse Zinthu 5 * 50mg / chinthu, kapena kuchuluka kwina |
★ Kuchuluka kwa substrate.
★ Kusintha kwakukulu.
★ Zogulitsa zochepa.
★ Zinthu zofatsa zomwe zimachitika chifukwa cha kukwiya.
★ Yosamalira chilengedwe.
➢ Kawirikawiri, njira yochitira zinthu iyenera kukhala ndi substrate, buffer solution ndi ES-AOX, ndipo mpweya uyenera kukhalapo.
➢ Mitundu yonse ya ES-AOXs yogwirizana ndi mikhalidwe yosiyanasiyana yabwino yochitira, yomwe ingaphunziridwe payekhapayekha.
➢ Kuchuluka kwa substrate kapena mankhwala omwe ali ndi substrate kungalepheretse ntchito ya ES-AOX. Komabe, kuletsa kumeneku kungachepetsedwe powonjezera substrate.
➢ Kusonkhanitsidwa kwa H2O2Mu dongosololi kungayambitse kuletsa kwa ma enzyme, komwe kungathetsedwe bwino pogwiritsa ntchito catalase.
Chitsanzo 1 (Kusungunuka kwa mowa wa aryl)(1):
Chitsanzo 2 (Kusungunuka kwa mafuta m'ma alcohols)(2):
Sungani kwa zaka ziwiri pansi pa -20℃.
Musagwirizane ndi zinthu zoopsa monga: kutentha kwambiri, pH yapamwamba/yotsika komanso organic solvent yochuluka.
1. Benen J AE, Sa' nchez-Torres P, Wagemaker M JM, ndi tal. J Biol Chem, 1998, 273(14): 7865-7872.
2. Mauersberger S, Drechsler H, Oehme G, ndi tal. Appl Microbiol Biot, 1992, 37: 66-73.
