Nucloside phosphorylse (NP)

Kufotokozera Kwachidule:

About nucleoside phosphorylse

Pali mitundu itatu ya zinthu zopangidwa ndi enzyme ya NP (Nambala monga ES-NP-101~ ES-NP-103) zomwe zapangidwa ndi SyncoZymes. ES-NP-101 ndi purine nucleoside phosphorylase, ES-NP-102 ndi ES-NP-103 ndi pyrimidine nucleoside phosphorylase. Nucleosidephosphorylase imatha kuwononga ma nucleosides kukhala maziko ndi pentose phosphate. Nucleoside phosphorylase ikhoza kugawidwa m'magulu awiri: purine nucleoside phosphorylase ndi pyrimidine nucleoside phosphorylase malinga ndi zomwe ma nucleoside bases amakonda. Purine nucleoside phosphorylase imatha kusintha adenosine kukhala adenine, inosine kukhala hypoxanthine, guanosine kukhala guanine, ndikupanga ribose phosphate nthawi imodzi. Pyrimidine nucleoside phosphorylase imatha kusintha uridine kukhala uracil ndikupanga ribose phosphate.

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Tsatanetsatane wa Zamalonda

Ma tag a Zamalonda

Mtundu wa reaction wa nucloside phosphorylse:

Zambiri Zamalonda:

Ma enzyme	Khodi ya Zamalonda	Kufotokozera
Ufa wa Enzyme	ES-NP-101~ ES-NP-103	seti ya 3 Nuclosidephosphorylse, 50 mg iliyonse 3 zinthu * 50mg / chinthu, kapena kuchuluka kwina

Njira yowunikira zochita za Nucloside phosphorylse:

Njira yotsatirayi yochitira zinthu ingagwiritsidwe ntchito pofotokoza:
Gawo la 1-10 mg/ml
Enzyme ya 10 mg/ml
100 mM Phosphate buffer (pH7.0)
Ikani zinthuzo mu chivundikiro cha kutentha chokhazikika (monga 30oC, 150rpm) kwa maola 12 mpaka 24. Ikani mu centrifuge chisakanizo chilichonse kuti chikhale ndi mapuloteni okwanira, ndipo supernatant ingagwiritsidwe ntchito pa TLC, HPLC kapena GC analysis.