Nucloside phosphoryalse (NP)

Kufotokozera Kwachidule:

Za nucleoside phosphoryalse

Pali mitundu itatu ya zinthu za NP enzyme (Nambala monga ES-NP-101~ ES-NP-103) yopangidwa ndi SyncoZymes.ES-NP-101 ndi purine nucleoside phosphorylase, ES-NP-102 ndi ES-NP-103 ndi pyrimidine nucleoside phosphorylase.Nucleosidephosphorylase imatha kuwola ma nucleoside kukhala maziko ndi pentose phosphate.Nucleoside phosphorylase akhoza kugawidwa mu purine nucleoside phosphorylase ndi pyrimidine nucleoside phosphorylase malinga ndi zokonda za maziko nucleoside.Purine nucleoside phosphorylase imatha kusokoneza adenosine kukhala adenine, inosine kupita ku hypoxanthine, guanosine kupita ku guanine, ndikupanga ribose phosphate nthawi yomweyo.Pyrimidine nucleoside phosphorylase imatha kusokoneza uridine ku uracil ndikupanga ribose phosphate.

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Titumizireni imelo

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Nucloside phosphoryalse reaction mtundu:

Zambiri Zamalonda:

Ma enzyme	Kodi katundu	Kufotokozera
Enzyme Powder	ES-NP-101~ ES-NP-103	seti ya 3 Nuclosidephosphoryalse, 50 mg chilichonse 3 zinthu * 50mg / chinthu, kapena kuchuluka kwina

Njira zowunikira za Nucloside phosphoryalse:

Njira zotsatirazi zitha kugwiritsidwa ntchito pofotokoza:
1-10 mg / ml gawo lapansi
10 mg/ml enzyme
100 mM Phosphate buffer (pH7.0)
Ikani zomwe zikuchitika pa kutentha kosasinthasintha (mwachitsanzo, 30oC, 150rpm) kwa maola 12-24.Centrifuge chosakaniza chilichonse kuti chiwongolere mapuloteni, iwo omwe ali apamwamba amatha kugwiritsidwa ntchito pakuwunika kwa TLC, HPLC kapena GC.