Lactate Dehydrogenase (LDH)
Ma enzyme | Kodi katundu | Kufotokozera |
Enzyme Powder | ES-LDH-101 | seti ya 1 Monoamine Oxidase, 50 mg chilichonse 1 zinthu * 50mg / chinthu, kapena kuchuluka kwina |
Njira zotsatirazi zitha kugwiritsidwa ntchito pofotokoza:
1-10 mg/ml: gawo lapansi
10 mg/ml: Enzyme
10% (v/v): organic cosolvent (1)
100 mM: Phosphate buffer (pH7.0)
0.2 mg/ml: NAD+
100 mM: Ammonium formate
10 mg/ml: Pangani dehydrogenase (FDH)
Ikani zomwe zikuchitika pa kutentha kosasinthasintha (mwachitsanzo, 30oC, 150rpm) kwa maola 24-48.Centrifuge chosakaniza chilichonse kuti chiwongolere mapuloteni, iwo omwe ali apamwamba amatha kugwiritsidwa ntchito pakuwunika kwa TLC, HPLC kapena GC.
Zindikirani (1): Zosungunulira za organic (monga DMSO, methanol, acetonitrile, IPA, etc.) ndizosankha, zomwe zimagwiritsidwa ntchito popanga magawo osasungunuka.
Lembani uthenga wanu apa ndikutumiza kwa ife