Ketoreductase (KRED)
KRED kuchokera ku SyncoZymes: Pali mitundu 191 ya zinthu za KRED enzyme (Nambala monga ES-KRED-101~ES-KRED-291) yopangidwa ndi SyncoZymes. SZ-KRED ingagwiritsidwe ntchito kwambiri pochepetsa ma aldehydes, ma beta-ketone esters, ma α-ketone esters ndi ma ketones m'chigawo ndi m'mbali.
Mtundu wa catalytic reaction:
| Ma enzyme | Khodi ya Zamalonda | Kufotokozera |
| Ufa wa Enzyme | Ufa wa Enzyme | seti ya ma Ketoreductase 187, 50 mg iliyonse, zinthu 187 * 50mg / chinthu, kapena kuchuluka kwina |
| Zida Zowunikira (SynKit) | ES-KRED-18100 | seti ya ma Ketoreductase 181, 1 mg iliyonse Zinthu 181 * 1mg / chinthu |
★ Kuchuluka kwa substrate.
★ Kusankha bwino kwa chiral.
★ Kutembenuza bwino kwambiri.
★ Zogulitsa zochepa.
★ Zinthu zofatsa zomwe zimachitika chifukwa cha kukwiya.
★ Yosamalira chilengedwe.
➢ Kuwunika ma enzyme kuyenera kuchitika pa ma substrate enaake chifukwa cha kudziwika kwa substrate, ndikupeza enzyme yomwe imayambitsa substrate yomwe ikufunidwa ndi zotsatira zabwino kwambiri zoyambitsa.
➢ Musagwirizane ndi zinthu zoopsa monga: kutentha kwambiri, pH yapamwamba/yotsika komanso organic solvent yokhala ndi kuchuluka kwakukulu.
➢ Kawirikawiri, njira yochitira zinthu iyenera kukhala ndi substrate, buffer solution, coenzymes (NAD(H) kapena NADP(H)), coenzyme regeneration system (monga shuga ndi glucose dehydrogenase).
➢ KRED iyenera kuwonjezeredwa komaliza mu dongosolo la reaction, pH ndi kutentha zitasinthidwa kuti zigwirizane ndi momwe reaction imakhalira.
➢ Mitundu yonse ya KRED ili ndi zinthu zosiyanasiyana zomwe zingathandize kwambiri pakuchitapo kanthu, choncho iliyonse iyenera kuphunziridwa payekhapayekha.
Chitsanzo 1(1):
Chitsanzo 2(2):
1. Li Z, Liu WD, Chen X, ndi tal. Tetrahedron, 2013, 69, 3561-3564.
2. Ni Y, Li CX, Ma HM, e tal. Appl Microbiol Biotechnol, 2011, 89: 1111-1118.
