Hydroaminase (HAM)
Kufotokozera Kwachidule:
Zokhudza Hydroaminase
Pali mitundu iwiri ya mankhwala a HAM enzyme (Nambala monga ES-HAM-101~ ES-HAM-102) yopangidwa ndi SyncoZymes. HAM imatha kuyambitsa ammonia ya enoic acid kapena zotumphukira zake kuti ipange chiral amino acid. HAM ingagwiritsidwe ntchito kupanga chiral amino acid (kapena chiral amines) kuchokera ku enoic acids (kapena alkenes). Wopereka ammonia, monga madzi a ammonia kapena mchere wa ammonium, ndi wofunikira.
Hydroaminase catalytic reaction mtundu
Foni yam'manja/Wechat/WhatsApp: +86-13681683526
Imelo:lchen@syncozymes.com
| Ma enzyme | Khodi ya Zamalonda | Kufotokozera |
| Ufa wa Enzyme | ES-HAM-101~ ES-HAM-102 | seti ya 2 Nuclosidephosphorylse, 50 mg iliyonse 2 zinthu * 50mg / chinthu, kapena kuchuluka kwina |
Njira yotsatirayi yochitira zinthu ingagwiritsidwe ntchito pofotokoza:
1-10 mg/ml: Gawo lapansi
10 mg/ml: Enzyme
10% (v/v): Chosungunuka chachilengedwe (1)
10% (v/v): Amonia
Sinthani pH kufika pa 9.0 musanawonjezere enzyme, ndipo enzyme iyenera kuwonjezeredwa pamapeto pake. Ikani zomwe zimachitika pa kutentha kosalekeza (monga 30oC, 150rpm) kwa maola 24 mpaka 48. Ikani centrifuge chisakanizo chilichonse kuti chikhale ndi mapuloteni okwanira, ndipo supernatant ingagwiritsidwe ntchito pa TLC, HPLC kapena GC analysis.
Chidziwitso (1): Zosungunulira zachilengedwe (monga DMSO, methanol, acetonitrile, IPA, ndi zina zotero) ndizosankha, zomwe zimagwiritsidwa ntchito pazinthu zosasungunuka.
