Esterase ndi Lipase (PLE ndi CALB)
Pali mitundu 26 ya zinthu za PLE enzyme (Nambala monga ES-PLE-101 ~ ES-PLE-126) yopangidwa ndi SyncoZymes. ES-PLE ingagwiritsidwe ntchito pochotsa ma hydrolysis a aliphatic ndi ester compounds, kapena regioselective ndi stereoselective resolution kuti ipange chiral acids ndi zotumphukira zake.
Mtundu wa catalytic reaction:
★ Kuchuluka kwa substrate.
★ Kusankha bwino kwa chiral.
★ Kusintha kwakukulu.
★ Zogulitsa zochepa.
★ Zinthu zofatsa zomwe zimachitika chifukwa cha kukwiya.
★ Yosamalira chilengedwe.
➢ Kawirikawiri, njira yochitira zinthu iyenera kukhala ndi substrate, buffer solution ndi ES-PLE. Kuyezetsa kwa ma ES-PLE ena kumachitika mu gawo lachilengedwe.
➢ Mitundu yonse ya ma ES-PLE omwe akugwirizana ndi machitidwe osiyanasiyana abwino ayenera kuphunziridwa payekhapayekha.
Chitsanzo 1 (Biosynthesis ya Pregabalin Intermediate)(1):
Chitsanzo 2(2):
Chitsanzo 3(3):
Chitsanzo 4(4):
Sungani kwa zaka ziwiri pansi pa -20℃.
Musagwirizane ndi zinthu zoopsa monga: kutentha kwambiri, pH yapamwamba/yotsika.
1. Xu FX, Chen SY, Xu G, e tal. Appl. Biotechnol Bioproc E, 1988, 54(4): 1030.
2. Huang, FC, Lee, LF, Mittal, RSD e tal. J. Am. Chem.Soc, 1975, 97, 4144.
3. Kielbasinski, P., Goralczyk, P., Mikolajczyk, M., e tal. Synlett, 1994, 127.
4. Gais, HJ, Griebel, C., Buschmann, H. Tetrahedron:Asymmetry 2000, 11, 917
