Ene reductase (ERED)
Ma ES-ERED amakonza mitundu yosiyanasiyana ya ma substrate chifukwa cha mitundu yosiyanasiyana ya ma substrate. Kawirikawiri, C=C ya ma α, β-unsaturated compounds okhala ndi magulu otenga ma electron (kuphatikizapo ketone aldehyde, magulu a nitro, ma carboxylic acid, ma esters, anhydride, ma lactones, ma imines, ndi zina zotero) amachepetsedwa mosavuta ndi ma ES-ERED, koma ma bond awiri osagwira ntchito satero.
Pali mitundu 46 ya zinthu zopangidwa ndi ma enzyme a ERED (Nambala monga ES-ERED-101~ES-ERED-146) zomwe zapangidwa ndi SyncoZymes.
Njira yothandizira:
| Ma enzyme | Khodi ya Zamalonda | Kufotokozera |
| Ufa wa Enzyme | ES-ERED-101~ ES-ERED-146 | seti ya 46 Ene Reductases, 50 mg iliyonse 46 zinthu * 50mg / chinthu, kapena kuchuluka kwina |
| Zida Zowunikira (SynKit) | ES-ERED-4600 | seti ya 46 Ene Reductases, 50 mg iliyonse 46 zinthu * 50mg / chinthu, kapena kuchuluka kwina |
★ Kuchuluka kwa substrate.
★ Kusankha bwino kwa chiral.
★ Kusintha kwakukulu.
★ Zogulitsa zochepa.
★ Zinthu zofatsa zomwe zimachitika chifukwa cha kukwiya.
★ Yosamalira chilengedwe.
★ Chitetezo chapamwamba.
➢ Kawirikawiri, dongosolo la reaction liyenera kukhala ndi substrate, buffer solution (optimum reaction pH), coenzymes (NAD(H) kapena NADP(H) ), coenzyme regeneration system (monga glucose ndi glucose dehydrogenase) ndi ES-ERED.
➢ Ma ES-ERED onse akhoza kuyesedwa motsatana mu njira yochitira zinthu pamwambapa kapena ndi ERED Screening Kit (SynKit ERED).
➢ Mitundu yonse ya ma ES-ERED omwe ali ndi machitidwe osiyanasiyana abwino kwambiri ayenera kuphunziridwa payekhapayekha.
➢ Kuchuluka kwa substrate kapena mankhwala omwe ali ndi substrate kungalepheretse ntchito ya ES-ERED. Komabe, kuletsa kumeneku kungachepetsedwe powonjezera substrate.
Chitsanzo 1(α,β-unsaturated aldehydes kapena ketones)(1):
Chitsanzo 2(α,β-unsaturated carboxylic acids ndi zotumphukira zake)(2):
Sungani kwa zaka ziwiri pansi pa -20℃.
Musagwirizane ndi zinthu zoopsa monga: kutentha kwambiri, pH yapamwamba/yotsika komanso organic solvent yochuluka.
1. Lucídio C, Fardelone J, Augusto R, ndi tal. J.Mol.Catal.B:Enzym., 2004, 29: 41-45.
2. Stueckler C, Hall M, Ehammer H, etal. .Org.Lett, 2007, 9(26): 5409-5411.
