Cyclohexanone monooxygenase (CHMO)
Kufotokozera Kwachidule:
Zokhudza Cyclohexanone monooxygenase
ES-CHMO (Cyclohexanone monooxygenase): Baeyer Villiger monooxygenase (BVMO) yokhala ndi cyclohexanone ngati substrate, imatha kupangitsa cyclohexanone kukhala cyclohexanol pogwiritsa ntchito mpweya.
Ndi mtundu wa enzyme yomwe imadalira flavin. Flavin yosungunuka imachepetsedwa kukhala flavin kudzera mu kayendedwe ka kuchepetsa coenzyme II (NADPH) kuti iwonongeke. Chifukwa chake, cofactor NADPH ndiyofunikira mu kayendedwe ka flavin. Pali mtundu umodzi wokha wa enzyme ya CHMO (Nambala monga ES-CHMO101) yomwe idapangidwa ndi SyncoZymes, yomwe ingagwiritsidwe ntchito kupangitsa cyclohexanone kukhala cyclohexanone.
Mtundu wa catalytic reaction:
Foni yam'manja/Wechat/WhatsApp: +86-13681683526
Imelo:lchen@syncozymes.com
| Ma enzyme | Khodi ya Zamalonda | Kufotokozera |
| Zida Zowunikira (SynKit) | ES-CHMO-101 | seti ya 1 Ketoreductases, 1 mg iliyonse 1 mg iliyonse * 1 mg / chinthu |
★ Kuchuluka kwa substrate.
★ Kusankha bwino kwa chiral.
★ Kusintha kwakukulu.
★ Zogulitsa zochepa.
★ Zinthu zofatsa zomwe zimachitika chifukwa cha kukwiya.
★ Yosamalira chilengedwe.
➢ Kawirikawiri, njira yochitira zinthu iyenera kukhala ndi gawo lapansi, yankho la buffer, enzyme, coenzyme ndi coenzyme regeneration system (monga shuga ndi glucose dehydrogenase).
➢ CHMO iyenera kuwonjezeredwa komaliza mu dongosolo la reaction, pH ndi kutentha zitasinthidwa kuti zigwirizane ndi momwe reaction imakhalira.
Chitsanzo 1 (Kupanga kwa cyclohexanol kuchokera ku cyclohexanol)(1):
Sungani kwa zaka ziwiri pansi pa -20℃.
Musagwirizane ndi zinthu zoopsa monga: kutentha kwambiri, pH yapamwamba/yotsika komanso organic solvent yochuluka.
1. Friso, Marco, Appl Microbiol Biotechnol, 2017, 101(20): 7557-7565
