Amine Dehydrogenase (AmDH)
Kufotokozera Kwachidule:
Zokhudza Amine Dehydrogenase
ES-AmDH (Amine Dehydrogenase): enzyme yomwe ingathandize kupanga ammoniation ya gulu la carbonyl ndikulichepetsa kukhala gulu la amino. Ndi enzyme yodalira coenzyme NADH, ndipo NADH imagwira ntchito ngati chotumizira hydrogen. Pali mitundu inayi ya zinthu za enzyme ya AmDH (Nambala monga ES-AmDH101 ~ ES-AmDH104) yopangidwa ndi SyncoZymes, yomwe ingagwiritsidwe ntchito mu regioselective kapena stereoselective reductive amination ya aldehydes ndi ketones.
Mtundu wa catalytic reaction:
Foni yam'manja/Wechat/WhatsApp: +86-13681683526
Imelo:lchen@syncozymes.com
★ Kuchuluka kwa substrate.
★ Kusankha bwino kwa chiral.
★ Kusintha kwakukulu.
★ Zogulitsa zochepa.
★ Zinthu zofatsa zomwe zimachitika chifukwa cha kukwiya.
★ Yosamalira chilengedwe.
➢ Kawirikawiri, njira yochitira zinthu iyenera kukhala ndi gawo lapansi, yankho la buffer, enzyme, coenzyme ndi coenzyme regeneration system (monga shuga ndi glucose dehydrogenase).
➢ AmDH iyenera kuwonjezeredwa komaliza mu dongosolo la reaction, pH ndi kutentha zitasinthidwa kuti zigwirizane ndi momwe reaction imakhalira.
Chitsanzo 1 (Kupanga ma chiral amines ofanana ndi kuchepetsa mphamvu ya alkyl aryl ketones)(1):
Sungani kwa zaka ziwiri pansi pa -20℃.
Musagwirizane ndi zinthu zoopsa monga: kutentha kwambiri, pH yapamwamba/yotsika komanso organic solvent yochuluka.
1. Kong W, Liu Y, Huang C, et al. Angewandte Chemie International Edition, 2022: e202202264.
