Amidase (AMD)
Ma enzyme:Ndi ma macromolecular biological catalysts, ma enzymes ambiri ndi mapuloteni
Amidase:Catalyze the hydrolysis of osiyanasiyana amkati ndi akunja aliphatic ndi onunkhira amides mwa kusamutsa gulu acyl madzi ndi kupanga ufulu zidulo ndi ammonia.Ma hydroxamic acid ndi ma organic acids amagwiritsidwa ntchito kwambiri ngati mankhwala chifukwa ndizomwe zimayambitsa kukula, maantibayotiki ndi zoletsa zotupa.Ma amidase amatha kugawidwa mu mtundu wa R ndi mtundu wa S acylases malinga ndi chothandizira stereoselectivity.
Kuphatikiza pa kulimbikitsa hydrolysis ya ma amides, amidase amathanso kuyambitsa kusintha kwa acyl pamaso pa ma co-substrates monga hydroxylamine.
Amidase okhala ndi magwero osiyanasiyana ali ndi mawonekedwe a gawo lapansi losiyana, ena aiwo amatha kungotulutsa ma amide onunkhira, ena aiwo amatha kungotulutsa ma aliphatic amides, ndi ena hydrolyze α-kapena ω-amino amides.Ma amine ambiri amakhala ndi ntchito yabwino yothandizira ma acyclic kapena osavuta onunkhira amidi, koma pazovuta zonunkhira, ma heterocyclic amides, makamaka ma amide okhala ndi ortho substituents, nthawi zambiri amakhala otsika (ma enzyme ochepa okha ndi omwe amawonetsa zotsatira zabwino).
Makina a Catalytic:
| Ma enzyme | Kodi katundu | Kodi katundu |
| Enzyme Powder | ES-AMD-101~ ES-AMD-119 | seti ya 19 amidases, 50 mg chilichonse 19 zinthu * 50mg / chinthu, kapena kuchuluka kwina |
| Screening Kit (SynKit) | ES-AMD-1900 | gulu la 19 amidases, 1 mg aliyense 19 zinthu * 1mg / chinthu |
★ Kukhazikika kwa gawo lapansi.
★ Kusankha kwamphamvu kwachiral.
★ High kutembenuka bwino.
★ Zogulitsa zochepa.
★ Mkhalidwe wofatsa.
★ Okonda zachilengedwe.
➢ Kuwunika kwa enzyme kuyenera kuchitidwa pazagawo zinazake chifukwa cha kukhazikika kwa gawo lapansi, ndikupeza puloteni yomwe imathandizira gawo la chandamale lomwe lili ndi mphamvu yothandiza kwambiri.
➢ Osakhudzana ndi zinthu zowopsa monga: kutentha kwambiri, kutsika / kutsika pH ndi zosungunulira za organic zokhazikika kwambiri.
➢ Nthawi zambiri, kachitidwe kachitidwe kayenera kukhala gawo lapansi, yankho la bafa (The optimum reaction pH of enzyme).Ma co-substrates monga hydroxylamine ayenera kukhalapo mu acyl transfer reaction system.
➢ AMD ayenera kuwonjezeredwa komaliza mu anachita dongosolo ndi momwe akadakwanitsira anachita pH ndi kutentha.
➢ Mitundu yonse ya AMD imakhala ndi machitidwe abwino osiyanasiyana, kotero iliyonse iyenera kuphunziridwanso payekha.
Chitsanzo 1(1):
Ntchito ya Hydrolysis kupita ku magawo osiyanasiyana a Amide
| Gawo lapansi | Zochitika zenizeni μmol min-1mg-1 | Gawo lapansi | Zochitika zenizeni μmol min-1mg-1 |
| Acetamide | 3.8 | ο-OH benzamide | 1.4 |
| Propionamide | 3.9 | p-OH benzamide | 1.2 |
| Lactamide | 12.8 | ο-NH2benzamide | 1.0 |
| Butyramid | 11.9 | p-NH2benzamide | 0.8 |
| Isobutyramide | 26.2 | ο- Toluamide | 0.3 |
| Pentanamide | 22.0 | p- Toluamide | 8.1 |
| Hexanamide | 6.4 | Nicotinamide | 1.7 |
| Cyclohexanamide | 19.5 | Isonicotinamide | 1.8 |
| Acrylamide | 10.2 | Picolinamide | 2.1 |
| Metacrylamide | 3.5 | 3-Phenylpropionamide | 7.6 |
| Prolinamide | 3.4 | Indol-3-acetamide | 1.9 |
| Benzamide | 6.8 |
Zomwe zidachitika mu 50mM sodium phosphate buffer solution, pH 7.5, pa 70 ℃.
| Amidi | Hydroxylamine | Hydrazine |
| Acetamide | 8.4 | 1.4 |
| Propionamide | 18.4 | 3.0 |
| Isobutyramide | 25.0 | 22.7 |
| Benzamide | 9.2 | 6.1 |
Zomwe zidachitika mu 50mM sodium phosphate buffer solution, pH 7.5, pa 70 ℃.
Zogwirizana reagent ndende: amides, 100 mm (benzamide, 10 mm);hydroxylamine ndi hydrazine, 400 mmM;enzyme 0.9 μM
Chitsanzo 2(2):
Chitsanzo 3(3):
1. D'Abusco AS, Ammendola S., et al.Extremophiles, 2001, 5:183-192.
2. Guo FM, Wu JP, Yang LR, et al.Njira Biochemistry, 2015, 50 (8): 1400-1404.
3. Zheng RC, Jin JQ, Wu ZM, et al.Bioorganic Chemistry, 2017, Ikupezeka pa intaneti 7.
