Transaminase (ATA)
Ma enzyme: Ndi ma macromolecular biological catalysts, ma enzyme ambiri ndi mapuloteni.
Ma Transaminase: Gulu la ma enzyme omwe amalimbikitsa kusamutsa ma amino acid pakati pa ma amino acid ndi ma keto acid. Ma Transaminase ndi ma enzyme ofunikira kwambiri pakupanga ma chiral amines osafanana komanso kusinthika kwa mitundu yosiyanasiyana.
Aminotransferase ingagawidwe m'magulu anayi malinga ndi momwe zinthu zilili: Ⅰ, Ⅱ, Ⅲ ndi Ⅳ. Ma ω-aminotransferase ndi a transaminases a kalasi II, omwe nthawi zambiri amagwiritsidwa ntchito popanga ma chiral amines ndi ma amino acid osazolowereka, monga ma β-amino acid.
ω-aminotransferases: Nthawi zambiri, ω-transaminase imatanthauza gulu la ma enzyme, omwe amachititsa kuti ammonia isamutsidwe popanda α-amino acid ngati gawo kapena chinthu.
Njira yothandizira:
| Ma enzyme | Khodi ya Zamalonda | Khodi ya Zamalonda |
| Ufa wa Enzyme | ES-ATA-101~ ES-ATA-165 | seti ya 65 ω-Transaminases, 50 mg iliyonse 65 zinthu * 50mg / chinthu, kapena kuchuluka kwina |
| Zida Zowunikira (SynKit) | ES-ATA-6500 | seti ya 65 ω-Transaminases, 1 mg iliyonse Zinthu 65 * 1mg / chinthu |
★ Kuchuluka kwa substrate.
★ Kusankha bwino kwa chiral.
★ Kutembenuza bwino kwambiri.
★ Zogulitsa zochepa.
★ Zinthu zofatsa zomwe zimachitika chifukwa cha kukwiya.
★ Yosamalira chilengedwe.
➢ Kuwunika ma enzyme kuyenera kuchitika pa ma substrate enaake chifukwa cha kudziwika kwa substrate, ndikupeza enzyme yomwe imayambitsa substrate yomwe ikufunidwa ndi zotsatira zabwino kwambiri zoyambitsa.
➢ Musagwirizane ndi zinthu zoopsa monga: kutentha kwambiri, pH yapamwamba/yotsika komanso organic solvent yokhala ndi kuchuluka kwakukulu.
➢ Kawirikawiri, njira yochitira zinthu iyenera kukhala ndi substrate, buffer solution, amino donor (monga amino acids ndi 1-phenyl ethylamine) kapena receptor (monga keto acids), coenzyme (PLP), cosolvent (monga DMSO).
➢ ATA iyenera kuwonjezeredwa komaliza mu dongosolo la reaction, pH ndi kutentha zitasinthidwa kuti zigwirizane ndi momwe reaction ilili.
➢ Mitundu yonse ya ATA ili ndi zinthu zosiyanasiyana zomwe zingathandize kwambiri pakuchitapo kanthu, choncho iliyonse iyenera kuphunziridwa payekhapayekha.
Chitsanzo 1 (kupanga Sitagliptin, kupanga kosagwirizana)(1):
Chitsanzo 2 (Mexiletine, Kuphatikiza kwa kinetic resolution ndi asymmetric synthesis)(2):
1 Savile CK, Janey JM, Mundorff EC, ndi ena. Science, 2010, 329(16), 305-309.
2 Koszelewski D, Pressnitz D, Clay D, et al. Makalata achilengedwe, 2009,11(21):4810-4812.
