Transaminase (ATA)
Ma Enzymes: Ndi ma macromolecular biological catalysts, ma enzymes ambiri ndi mapuloteni.
Transaminase: Gulu la michere yomwe imathandizira kusamutsa kwa amino pakati pa amino acid ndi keto acid.Transaminase ndi ma enzymes ofunikira achilengedwe mu kaphatikizidwe ka asymmetric ndikusintha kwamtundu wa chiral amines.
Aminotransferase akhoza kugawidwa m'magulu anayi motsatira ndondomeko ndi kapangidwe: Ⅰ, Ⅱ, Ⅲ ndi Ⅳ.Ma ω-aminotransferases ali m'gulu la II transaminases, omwe amagwiritsidwa ntchito popanga ma amines a chiral ndi ma amino acid osakhala achilengedwe, monga ma β-amino acid.
ω-aminotransferases: Nthawi zambiri, ω-transaminase amatanthauza gulu la michere, yomwe imathandizira kusintha kwa ammonia popanda α-amino acid monga gawo lapansi kapena mankhwala.
Makina a Catalytic:
| Ma enzyme | Kodi katundu | Kodi katundu |
| Enzyme Powder | ES-ATA-101~ ES-ATA-165 | seti ya 65 ω-Transaminases, 50 mg chilichonse 65 zinthu * 50mg / chinthu, kapena kuchuluka kwina |
| Screening Kit (SynKit) | ES-ATA-6500 | seti ya 65 ω-Transaminases, 1 mg chilichonse 65 zinthu * 1mg / chinthu |
★ Kukhazikika kwa gawo lapansi.
★ Kusankha kwamphamvu kwachiral.
★ High kutembenuka bwino.
★ Zogulitsa zochepa.
★ Mkhalidwe wofatsa.
★ Okonda zachilengedwe.
➢ Kuwunika kwa enzyme kuyenera kuchitidwa pazagawo zinazake chifukwa cha kukhazikika kwa gawo lapansi, ndikupeza puloteni yomwe imathandizira gawo la chandamale lomwe lili ndi mphamvu yothandiza kwambiri.
➢ Osakhudzana ndi zinthu zowopsa monga: kutentha kwambiri, kutsika / kutsika pH ndi zosungunulira za organic zokhazikika kwambiri.
➢ Kawirikawiri, kachitidwe kachitidwe kamayenera kukhala ndi gawo lapansi, yankho la buffer, amino donor (monga amino acid ndi 1-phenyl ethylamine) kapena receptor (monga keto acids), coenzyme (PLP), cosolvent (monga DMSO).
➢ ATA iyenera kuwonjezeredwa komaliza mu machitidwe, pambuyo pa pH ndi kutentha kusinthidwa ku momwe zimakhalira.
➢ Mitundu yonse ya ATA ili ndi machitidwe abwino osiyanasiyana, kotero iliyonse iyenera kuphunziridwanso payekha.
Chitsanzo 1(kaphatikizidwe ka sitagliptin, kaphatikizidwe ka asymmetric)(1):
Chitsanzo 2 (Mexiletine, Kuphatikiza kwa kinetic resolution ndi asymmetric synthesis)(2):
1 Savile CK, Janey JM, Mundorff EC, et al.Sayansi, 2010, 329(16), 305-309.
2 Koszelewski D, Pressnitz D, Clay D, et al.Makalata achilengedwe, 2009,11(21):4810-4812.
