Phenylalanine dehydrogenase (PDH)
Kufotokozera Kwachidule:
About Phenylalanine dehydrogenase
ES-PDH: Gulu la oxidoreductase. Yankho labwino likhoza kuyambitsa kuchotsedwa kwa okosijeni kwa mchere wa L phenylalanine pamaso pa NAD kuti apange mchere wa phenylpyruvate, ndipo yankho lotsutsana nalo likhoza kuyambitsa kupanga mchere wa amino acid wofanana kuchokera ku mchere wa phenylpyruvate. Syncozymes adapanga zinthu 8 za phenylalanine dehydrogenase (yolembedwa ndi manambala a ES-PDH-101~ES-PDH-108). ES-PDH ingagwiritsidwe ntchito kukonza mchere wa phenylalanine kapena mchere wa amino acid wofanana kuchokera ku mchere wa phenylpyruvic acid kapena zotumphukira.
Mtundu wa catalytic reaction:
Foni yam'manja/Wechat/WhatsApp: +86-13681683526
Imelo:lchen@syncozymes.com
| Ma enzyme | Khodi ya Zamalonda | Kufotokozera |
| Ufa wa Enzyme | ES-PDH-101~ ES-PDH-108 | seti ya 8 Nitrile Reductase, 50 mg iliyonse 8 zinthu * 50mg / chinthu, kapena kuchuluka kwina |
★ Kuchuluka kwa substrate.
★ Kusankha bwino kwa chiral.
★ Kusintha kwakukulu.
★ Zogulitsa zochepa.
★ Zinthu zofatsa zomwe zimachitika chifukwa cha kukwiya.
★ Yosamalira chilengedwe.
➢ Kawirikawiri, njira yochitira zinthu iyenera kukhala ndi substrate, buffer solution, enzyme, coenzyme (NAD(H)), coenzyme regeneration system (monga ammonium formate ndi formate dehydrogenase).
➢ Mtundu uliwonse wa ES-PDH umagwirizana ndi machitidwe osiyanasiyana abwino kwambiri, omwe angaphunziridwe payekhapayekha ngati pakufunika.
➢ ES-PDH iyenera kuwonjezeredwa mu dongosolo lomaliza la reaction kuti ntchitoyo ipitirire.
Chitsanzo 1 (Kupanga zinthu kuchokera ku phenylalanine kuchokera ku phenylpyruvate derivatives)(1):
Sungani kwa zaka ziwiri pansi pa -20℃.
Musagwirizane ndi zinthu zoopsa monga: kutentha kwambiri, pH yapamwamba/yotsika komanso organic solvent yochuluka.
1. Francesca Paradisi, Stuart Collins, ndi ena. Journal of Biotechnology, 2007, 128, 408–411.
