Phenylalanine ammonia lyase (PAL)
Kufotokozera Kwachidule:
About Phenylalanine ammonia lyase
ES-PAL: Gulu la ma enzyme omwe amachititsa kuti L-phenylalanine ichotsedwe mwachindunji kukhala transcinnamic acid. Syncozymes adapanga zinthu 10 za phenylalanine ammonia lyase (yolembedwa ndi manambala a ES-PAL-101~ES-PAL-110), zomwe zingagwiritsidwe ntchito pochotsa kapena kubwezera phenylalanine ndi zotumphukira zake.
Mtundu wa catalytic reaction:
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Imelo:lchen@syncozymes.com
| Ma enzyme | Khodi ya Zamalonda | Kufotokozera |
| Ufa wa Enzyme | ES-PAL-101~ ES-PAL-110 | seti ya 10 L-phenylalanine ammonia-lyase, 50 mg iliyonse 10 zinthu * 50mg / chinthu, kapena kuchuluka kwina |
★ Kuchuluka kwa substrate.
★ Kusankha bwino kwa chiral.
★ Kusintha kwakukulu.
★ Zogulitsa zochepa.
★ Zinthu zofatsa zomwe zimachitika chifukwa cha kukwiya.
★ Yosamalira chilengedwe.
Kawirikawiri, njira yochitira zinthu iyenera kukhala ndi substrate, buffer solution ndi enzyme.
➢ Mtundu uliwonse wa ES-PAL umagwirizana ndi machitidwe osiyanasiyana abwino kwambiri, omwe angaphunziridwe payekhapayekha.
➢ ES-PAL iyenera kuwonjezeredwa mu dongosolo lochitapo kanthu kuti ntchitoyo ipitirire.
Chitsanzo 1 (Kupanganso kwa trans cinnamic acid kuchokera ku phenylalanine)(1):
Chitsanzo 2 (Kupanganso kwa phenylalanine kuchokera ku trans-cinnamic acid derivative)(2):
Sungani kwa zaka ziwiri pansi pa -20℃.
Musagwirizane ndi zinthu zoopsa monga: kutentha kwambiri, pH yapamwamba/yotsika komanso organic solvent yochuluka.
1. Zachary Mays, Karishma Mohan,ndi ena.Chem Commun (Camb).2020, 56, 5255-5258.
2. Ian Rowles, Bas Groenendaal,ndi ena. Tetrahedron,2016. 72, 7343-7347.
