Nitrile reductase (NRED)
Kufotokozera Kwachidule:
Za Nitrile reductase
Pali mitundu itatu ya mankhwala a enzyme ya NRED (Nambala monga ES-NRED101~ ES-NRED103) yopangidwa ndi SyncoZymes.NRED ikhoza kuthandizira kuchepetsa gulu la nitrile kukhala amine.M'kati mwa catalysis, NADPH imafunika ngati chonyamulira haidrojeni.
Nitrile reductasecatalytic reaction type
Mobile/Wechat/WhatsApp: +86-13681683526
Imelo:lchen@syncozymes.com
| Ma enzyme | Kodi katundu | Kufotokozera |
| Kufotokozera | ES- NRED -101~ ES- NRED -103 | seti ya 3 Nitrile Reductase, 50 mg chilichonse 3 zinthu * 50mg / chinthu, kapena kuchuluka kwina |
Njira zotsatirazi zitha kugwiritsidwa ntchito pofotokoza:
1-10 mg / ml gawo lapansi
10 mg/ml enzyme
10% (v/v) organic cosolvent (1)
100 mM Phosphate buffer (pH7.0)
0.2 mg/ml NADP+
10 mg / ml shuga
2 mg/ml Glucose dehydrogenase (GDH)
Ikani zomwe zikuchitika pa kutentha kosasinthasintha (mwachitsanzo, 30oC, 150rpm) kwa maola 24-48.Centrifuge aliyense osakaniza kuti precipitate mapuloteni, iwo supernatant angagwiritsidwe ntchito TLC, HPLC kapena GC kusanthula.
Zindikirani (1): Zosungunulira za organic (monga DMSO, methanol, acetonitrile, IPA, etc.) ndizosankha, zomwe zimagwiritsidwa ntchito popanga magawo osasungunuka.
