Nitrile reductase (NRED)
Kufotokozera Kwachidule:
Zokhudza Nitrile reductase
Pali mitundu itatu ya mankhwala a NRED enzyme (Nambala monga ES-NRED101~ ES-NRED103) yopangidwa ndi SyncoZymes. NRED imatha kuyambitsa kuchepa kwa gulu la nitrile kukhala amine. Mu catalysis, NADPH imafunika ngati chonyamulira hydrogen.
Nitrile reductasecatalytic reaction mtundu
Foni yam'manja/Wechat/WhatsApp: +86-13681683526
Imelo:lchen@syncozymes.com
| Ma enzyme | Khodi ya Zamalonda | Kufotokozera |
| Kufotokozera | ES- NRED -101~ ES- NRED -103 | seti ya 3 Nitrile Reductase, 50 mg iliyonse Zinthu zitatu * 50mg / chinthu, kapena kuchuluka kwina |
Njira yotsatirayi yochitira zinthu ingagwiritsidwe ntchito pofotokoza:
Gawo la 1-10 mg/ml
Enzyme ya 10 mg/ml
10% (v/v) Chosungunuka chachilengedwe (1)
100 mM Phosphate buffer (pH7.0)
0.2 mg/ml NADP+
10 mg/ml Shuga
2 mg/ml Glucose dehydrogenase (GDH)
Ikani zinthuzo mu chivundikiro cha kutentha chokhazikika (monga 30oC, 150rpm) kwa maola 24 mpaka 48. Ikani mu centrifuge chisakanizo chilichonse kuti chikhale ndi mapuloteni okwanira, ndipo supernatant ingagwiritsidwe ntchito pa TLC, HPLC kapena GC analysis.
Chidziwitso (1): Zosungunulira zachilengedwe (monga DMSO, methanol, acetonitrile, IPA, ndi zina zotero) ndizosankha, zomwe zimagwiritsidwa ntchito pazinthu zosasungunuka.
