Nitrile hydratase (NHT)
Kufotokozera Kwachidule:
Zokhudza ES-NHTs
ES-NHTs: gulu la metalloenzyme, lomwe limasankha ma nitriles kukhala ma primary amide ofanana. Amagwiritsidwa ntchito kwambiri osati kokha popanga ma amino acid, ma amide, ma carboxylic acid ndi zotumphukira zawo, komanso pokonzekera mankhwala a chiral. Pali mitundu 24 ya nitrile hydratases yomwe idapangidwa ndi SyncoZymes (Nambala monga ES-NHT-101 ~ ES-NHT-124).
Mtundu wa catalytic reaction:
Foni yam'manja/Wechat/WhatsApp: +86-13681683526
Imelo:lchen@syncozymes.com
| Ma enzyme | Khodi ya Zamalonda | Kufotokozera |
| Ufa wa Enzyme | ES-NHT-101~ ES-NHT-124 | seti ya ma Nitrile Hydratases 24, 50 mg iliyonse, zinthu 24 * 50mg / chinthu, kapena kuchuluka kwina |
| Zida Zowunikira (SynKit) | ES-NHT-2400 | seti ya ma Nnitrile Hydratases 24, 1 mg iliyonse Zinthu 24 * 1mg / chinthu |
★ Kuchuluka kwa substrate.
★ Kusankha bwino kwa chiral.
★ Kusintha kwakukulu.
★ Zogulitsa zochepa.
★ Zinthu zofatsa zomwe zimachitika chifukwa cha kukwiya.
★ Yosamalira chilengedwe.
➢ Kawirikawiri, njira yochitira zinthu iyenera kukhala ndi substrate, yankho la buffer (optimum reaction pH) ndi ES-NHT.
➢ Ma ES-NHT onse akhoza kuyesedwa motsatana mu njira yochitira zinthu pamwambapa kapena ndi NHT Screening Kit (SynKit NHT).
➢ Mitundu yonse ya ES-NHTs yogwirizana ndi machitidwe osiyanasiyana abwino kwambiri iyenera kuphunziridwa payekhapayekha.
➢ Kuchuluka kwa zinthu kapena zinthu zomwe zili ndi zinthuzi kungalepheretse ntchito ya ES-NHT. Komabe, kuletsa kumeneku kungachepetsedwe powonjezera zinthuzi, kuzilumikiza ndi ma amidase kapena kuchotsa zinthuzi.
Chitsanzo 1 (kugwirizana ndi amidase)(1):
Chitsanzo 2(2):
Sungani kwa zaka ziwiri pansi pa -20℃.
Musagwirizane ndi zinthu zoopsa monga: kutentha kwambiri, pH yapamwamba/yotsika komanso organic solvent yochuluka.
1 Vojtech V, Ludmila M, Alicja BV, ndi tal. J Mol Catal B-enzym, 2011, 71: 51-55.
2 Gary W. B, Thomas G, Christopher B. M, ndi tal. Tetrahedron lett, 2010, 51: 1639-1641.
