Nitrilase (NIT)
Kufotokozera Kwachidule:
About Nitrilase
ES-NITs: Sinthani mwachindunji gulu la cyanide kukhala gulu la carboxyl, kuti mukonzekere carboxylic acid, yomwe imagwiritsidwa ntchito kwambiri mu zamankhwala, kuteteza chilengedwe, makampani opanga mankhwala, zowonjezera chakudya, nsalu, ulimi ndi zina.
Pali mitundu 40 ya zinthu za Nitrilase (NIT) (Nambala monga ES-NIT-101~ES-NIT-140) yopangidwa ndi SyncoZymes. ES-NIT ndi chida chothandiza chothandizira kupanga chiral carboxylic acids kudzera mu regio- ndi stereoselective hydrolysis ya mitundu yosiyanasiyana ya aliphatic ndi aromatic nitriles.
Mtundu wa catalytic reaction:
Foni yam'manja/Wechat/WhatsApp: +86-13681683526
Imelo:lchen@syncozymes.com
| Ma enzyme | Khodi ya Zamalonda | Kufotokozera |
| Ufa wa Enzyme | ES-NIT-101~ ES-NIT-140 | seti ya 40 Nitrilase, 50 mg iliyonse Zinthu 40 * 50mg / chinthu, kapena kuchuluka kwina |
| Zida Zowunikira (SynKit) | ES-NIT-4000 | seti ya 40 Nitrilase, 1 mg iliyonse Zinthu 40 * 1mg / chinthu |
★ Kuchuluka kwa substrate.
★ Kusintha kwakukulu.
★ Zogulitsa zochepa.
★ Zinthu zofatsa zomwe zimachitika chifukwa cha kukwiya.
★ Yosamalira chilengedwe.
➢ Kawirikawiri, njira yochitira zinthu iyenera kukhala ndi substrate, yankho la buffer (pH yabwino kwambiri) ndi ES-NIT.
➢ Ma ES-NIT onse akhoza kuyesedwa motsatana mu dongosolo la reaction lomwe lili pamwambapa kapena ndi NIT Screening Kit (SynKit NIT).
➢ Mitundu yonse ya ES-NITs yogwirizana ndi machitidwe osiyanasiyana abwino kwambiri iyenera kuphunziridwa payekhapayekha.
➢ Kuchuluka kwa substrate kapena mankhwala omwe ali ndi substrate kungalepheretse ntchito ya ES-NIT. Komabe, kuletsako kungachepetsedwe powonjezera substrate.
Chitsanzo 1 (Kupanga nicotinic acid)(1):
Chitsanzo 2 (Kupangidwa kwa (R)-(-)-mandelic acid)(2):
Chitsanzo 3 (Kupanga baclofen)(3):
Chitsanzo 4 (Kupanga glycolic acid)(4):
Sungani kwa zaka ziwiri pansi pa -20℃.
Musagwirizane ndi zinthu zoopsa monga: kutentha kwambiri, pH yapamwamba/yotsika komanso organic solvent yochuluka.
1 Mathew CD, Nagasawa T, Kobayashi M, etal. Appl. Environ. Microbiol, 1988, 54(4): 1030.
2 Yamamoto K, Oishi K, Fujimatsu I, e tal. Appl. Kuzungulira. Microbiol, 1991, 57(10): 3028.
3 Xu MZ, Ren J, Gong JS, ndi tal. Chin. J. Biotechnol, 2013, 29(1): 31.
4 Wu S, Fogiel AJ, Petrillo KL, ndi tal. Biotechnol. Bioeng., 2008, 99(3): 717.
