Chithunzi Chodziwika cha CALB Chosayenda

CALB Yosayenda

Kufotokozera Kwachidule:

CALB

Lipase B yopangidwanso kuchokera ku Candida antarctica (CALB) imapangidwa pogwiritsa ntchito kuwiritsa pansi pa madzi pogwiritsa ntchito Pichia pastoris yosinthidwa majini.

CALB ingagwiritsidwe ntchito mu gawo la madzi kapena gawo lachilengedwe lothandizira esterification, esterolysis, transesterification, ring opening polyester synthesis, aminolysis, hydrolysis of amides, acylation of amines ndi addition reaction.

CALB ili ndi kusankha kwakukulu kwa chiral komanso kusankha malo, kotero ingagwiritsidwe ntchito kwambiri mu kukonza mafuta, chakudya, mankhwala, zodzoladzola ndi mafakitale ena a mankhwala.

Foni yam'manja/Wechat/WhatsApp: +86-13681683526

Imelo:lchen@syncozymes.com

Tumizani imelo kwa ife

Tsatanetsatane wa Zamalonda

Ma tag a Zamalonda

CALB Yosayenda:

CALB imalepheretsedwa kuyenda chifukwa cha kulowetsedwa kwa utomoni wonyowa kwambiri womwe ndi polymer wokhala ndi mapokoso ambiri, styrene/methacrylate. CALB yosasinthika ndi yoyenera kugwiritsidwa ntchito mu zosungunulira zachilengedwe ndi machitidwe opanda zosungunulira, ndipo imatha kubwezeretsedwanso ndikugwiritsidwanso ntchito nthawi zambiri m'mikhalidwe yoyenera.
Khodi Yogulitsa: SZ-CALB- IMMO100A, SZ-CALB- IMMO100B.

Ubwino wa SZ-CALB-IMMO100:

★Kuchita zinthu mopitirira muyeso, kusankha bwino kwa chiral komanso kukhazikika kwambiri.
★Kugwira ntchito bwino m'magawo osagwiritsa ntchito madzi.
★Chotsani mosavuta mu dongosolo la reaction, chotsani mwachangu reaction, ndipo pewani zotsalira za mapuloteni mu mankhwala.
★Ikhoza kubwezeretsedwanso ndikugwiritsidwanso ntchito kuti ichepetse mtengo.

Magawo a Zamalonda a SZ-CALB-IMMO100:

Ntchito	≥10000PLU/g
pH ya zomwe zimachitika	5-9
Kuchuluka kwa kutentha kwa zomwe zimachitika	10-60℃
Maonekedwe	CALB-IMMO100-A: Cholimba chachikasu chopepuka mpaka bulauni CALB-IMMO100-B: Cholimba choyera mpaka chofiirira chopepuka
Kukula kwa tinthu	300-500μm
Kutayika pakuuma pa 105℃	0.5% -3.0%
Utomoni woletsa kuyenda	Polima ya macroporous, styrene/methacrylate
Zosungunulira zoyankha	Madzi, zosungunulira zachilengedwe, ndi zina zotero, kapena zopanda zosungunulira. Pazochita mu zosungunulira zachilengedwe zina, madzi 3% akhoza kuwonjezeredwa kuti akonze zotsatira za kuchitapo kanthu
Kukula kwa tinthu	CALB-IMMO100-A: 200-800 μm CALB-IMMO100-B: 400-1200 μm

Tanthauzo la gawo: Gawo limodzi likugwirizana ndi kapangidwe ka 1μmol pa mphindi imodzi ya propyl laurate kuchokera ku lauric acid ndi 1-propanol pa 60℃. CALB-IMMP100-A ndi CALB-IMMO100-B zomwe zili pamwambapa zimagwirizana ndi zonyamulira zosayenda zomwe zili ndi kukula kosiyanasiyana kwa tinthu.

Malangizo ndi Zotetezera:

1. Mtundu wa reactor
Enzyme yosayenda bwino imagwiritsidwa ntchito pa kettle batch reactor komanso pa fixed bed continuous flow reactor. Izi ziyenera kukumbukiridwa kuti zisaphwanye chifukwa cha mphamvu yakunja panthawi yodyetsa kapena kudzaza.
2. pH ya reaction, kutentha ndi zosungunulira
Enzyme yosayenda iyenera kuwonjezeredwa pomaliza, zinthu zina zitawonjezeredwa ndi kusungunuka, ndipo pH yake iyenera kusinthidwa.
Ngati kugwiritsa ntchito substrate kapena kupangidwa kwa chinthucho kudzapangitsa kuti pH isinthe panthawi ya reaction, buffer yokwanira iyenera kuwonjezeredwa ku reaction system, kapena pH iyenera kuyang'aniridwa ndikusinthidwa panthawi ya reaction.
Munthawi yomwe kutentha kwa CALB kumayenderana ndi kutentha (pansi pa 60 ℃), kuchuluka kwa kusintha kunawonjezeka ndi kutentha komwe kunakwera. Pogwiritsidwa ntchito moyenera, kutentha kwa reaction kuyenera kusankhidwa malinga ndi kukhazikika kwa substrate kapena chinthucho.
Kawirikawiri, ester hydrolysis reaction ndi yoyenera mu aqueous phase system, pomwe ester synthesis reaction ndi yoyenera mu organic phase system. organic solvent ikhoza kukhala ethanol, tetrahydrofuran, n-hexane, n-heptane ndi toluene, kapena mixed solvent yoyenera. Pa reaction mu organic solvents zina, 3% ya madzi ikhoza kuwonjezeredwa kuti iwonjezere reaction effect.
3. Kugwiritsanso ntchito ndi nthawi yogwira ntchito ya CALB
Pansi pa momwe zinthu zilili zoyenera, CALB ikhoza kubwezeretsedwanso ndikugwiritsidwanso ntchito, ndipo nthawi yeniyeni yogwiritsira ntchito imasiyana malinga ndi mapulojekiti osiyanasiyana.
Ngati CALB yomwe yabwezedwayo sigwiritsidwanso ntchito nthawi zonse ndipo ikufunika kusungidwa ikabwezedwa, iyenera kutsukidwa, kuumitsidwa ndikutsekedwa pa kutentha kwa 2-8 ℃.
Pambuyo pobwerezabwereza kangapo, ngati mphamvu ya reaction yachepa pang'ono, CALB ikhoza kuwonjezeredwa moyenera ndikupitiliza kugwiritsidwa ntchito. Ngati mphamvu ya reaction yachepa kwambiri, iyenera kusinthidwa.

Zitsanzo za Kugwiritsa Ntchito:

Chitsanzo 1 (Aminolysis)⁽¹⁾:

Chitsanzo 2 (Aminolysis)⁽²⁾:

Chitsanzo 3 (Kupanga mphete ya polyester yotsegula mphete)⁽³⁾:

Chitsanzo 4 (Kusintha kwa ma hydroxyl kukhala ma transester, kusankha kwa regioselective)⁽⁴⁾:

Chitsanzo 5 (Kusintha kwa ma ester, kutsimikiza kwa kayendedwe ka mowa wa racemic alcohols)⁽⁵⁾:

Chitsanzo 6 (Esterification, kinetic resolution ya carboxylic acid)⁽⁶⁾:

Chitsanzo 7 (Esterolysis, kinetic resolution)⁽⁷⁾:

Chitsanzo 8 (Hydrolysis ya amides)⁽⁸⁾:

Chitsanzo 9 (Kuchuluka kwa ma amine)⁽⁹⁾:

Chitsanzo 10 (Njira yowonjezera ya Aza-Michael)⁽¹⁰⁾:

Maumboni:

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