Research Express |Kafukufuku waku University of Tsinghua akuwonetsa kuti NMN imatha kuchiza kutupa

Macrophage activation ndi njira ya pathogenic yomwe imayambitsa kutupa kosatha m'thupi , koma kupitilira kwa macrophage kungayambitse kutupa kosatha ndi matenda monga insulin kukana ndi matenda aakulu monga atherosclerosis.PGE 2, yomwe imagwirizanitsa kuyankha kotupa, imapangidwa kuchokera ku arachidonic acid ndi cyclooxygenases (COX-1 ndi COX-2).COX-1 ndi COX-2 ndizofunikira kwambiri za anti-kutupa ndipo zimatha kuletsedwa ndi mankhwala osagwiritsa ntchito steroidal anti-inflammatory drugs (NSAIDs).

kugwiritsa ntchito NSAIDs kumatha kuyambitsa zovuta zambiri, monga magazi m'mimba.Choncho, ndikofunikira kwambiri kupeza chinthu chotetezeka chachilengedwe chochizira kutupa .

Posachedwapa, gulu lofufuza lochokera ku yunivesite ya Tsinghua linachitira mbewa macrophages ndi NMN, ndipo linatsimikizira kupyolera muzoyesera kuti NMN ikhoza kuchepetsa kudzikundikira kwa mapuloteni okhudzana ndi kutupa ndi zowonongeka za kagayidwe kachakudya, ndikuletsa kuyankhidwa kwa kutupa kwa macrophages.Malire mu Molecular Bioscience .

Kutupa kumasintha milingo ya metabolic byproducts mu macrophages
Choyamba, gulu lofufuza lidayambitsa macrophages kuti apange kutupa kudzera mu lipopolysaccharide (LPS), ndikusanthula zomwe zili muzinthu zozungulira macrophages panthawi yotupa.Miyezo ya metabolites 99 idawonjezeka ndipo metabolites 105 idachepetsedwa pakati pa mamolekyu a 458 omwe adapezeka asanayambe komanso pambuyo pa kukondoweza kotupa, ndipo milingo ya NAD + yomwe imatsagana ndi kutupa idachepanso.

(CHITHUNZI 1)

NMN imawonjezera milingo ya NAD ndikuchepetsa kutupa kwa macrophage
Gulu lofufuzalo linachitiranso macrophages ndi LPS, zomwe zimapangitsa kuti thupi likhale lopweteka, IL-6 ndi IL-1β, ma cytokines oletsa kutupa omwe amakhala ngati zizindikiro za kutupa.Pambuyo pa chithandizo cha NMN cha LPS-induced macrophage inflammation, zinapezeka kuti mlingo wa NAD wa intracellular unawonjezeka ndipo mawu a mRNA a IL-6 ndi IL-1β anachepetsedwa.Mayesero adawonetsa kuti NMN idakulitsa kuchuluka kwa NAD ndikuchepetsa kutupa kwa macrophage kwa LPS.

(Chithunzi 2)

(Chithunzi 3)

NMN imachepetsa kuchuluka kwa mapuloteni okhudzana ndi kutupa
Kuchiza kwa NMN, kunapezeka kuti mapuloteni okhudzana ndi kutupa monga RELL1, PTGS2, FGA, FGB ndi igkv12-44 anachepetsedwa m'maselo, zomwe zimasonyeza kuti NMN inachepetsa kufotokoza kwa mapuloteni okhudzana ndi kutupa.

(Chithunzi 4)

NMN imachepetsa kufotokoza kwa mapuloteni a NSAIDS
Kuyesera komaliza kunapeza kuti NMN inachepetsa mlingo wa PGE2 mu LPS-activated RAW264.7 maselo mwa kuchepetsa mlingo wa mawu a COX-2, motero kuchepetsa mawu a COX2 ndi kuletsa kutupa kwa LPS.

(Chithunzi 6)

pomaliza, kuwonjezereka kwa NMN kumatha kuchiza bwino kutupa kosatha kwa mbewa, ndipo chithandizo cha kutupa kwa anthu chiyenera kutsimikiziridwa ndi mayesero oyenerera achipatala.Mwina NMN ilowa m'malo mwa NSAIDS posachedwa.

maumboni:
1.Liu J, Zong Z, Zhang W, Chen Y, Wang X, Shen J, Yang C, Liu X, Deng H. Nicotinamide Mononucleotide Amachepetsa Kutupa Kwambiri kwa LPS ndi Kupanikizika kwa Oxidative Kupyolera mu Kuchepetsa COX-2 Kufotokozera mu Macrophages.Front Mol Biosci.2021 Jul 6.